
Proceedings Paper
The multifunctional application of microfluidic lab-on-a-chip surface enhanced Raman spectroscopy (LOC-SERS) within the field of bioanalyticsFormat | Member Price | Non-Member Price |
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Paper Abstract
This contribution will present a variety of applications of lab-on-a-chip surface enhanced Raman spectroscopy in the
field of bioanalytic. Beside the quantification and online monitoring of drugs and pharmaceuticals, determination of
enzyme activity and discrimination of bacteria are successfully carried out utilizing LOC-SERS. The online-monitoring
of drugs using SERS in a microfluidic device is demonstrated for nicotine. The enzyme activity of thiopurine
methyltransferase (TPMT) in lysed red blood cells is determined by SERS in a lab-on-a-chip device. To analyse the
activity of TPMT the metabolism of 6-mercaptopurine to 6-methylmercaptopurine is investigated. The discrimination of
bacteria on strain level is carried out with different E. coli strains. For the investigations, the bacteria are busted by ultra
sonic to achieve a high information output. This sample preparation provides the possibility to detect SERS spectra
containing information of the bacterial cell walls as well as of the cytoplasm. This contribution demonstrates the great
potential of LOC-SERS in the field of bioanalytics.
Paper Details
Date Published: 8 June 2011
PDF: 9 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808707 (8 June 2011); doi: 10.1117/12.890086
Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)
PDF: 9 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808707 (8 June 2011); doi: 10.1117/12.890086
Show Author Affiliations
Anne März, Friedrich-Schiller-Univ. Jena (Germany)
Bettina Mönch, Friedrich-Schiller-Univ. Jena (Germany)
Angela Walter, Friedrich-Schiller-Univ. Jena (Germany)
Thomas Bocklitz, Friedrich-Schiller-Univ. Jena (Germany)
Bettina Mönch, Friedrich-Schiller-Univ. Jena (Germany)
Angela Walter, Friedrich-Schiller-Univ. Jena (Germany)
Thomas Bocklitz, Friedrich-Schiller-Univ. Jena (Germany)
Wilm Schumacher, Friedrich-Schiller-Univ. Jena (Germany)
Petra Rösch, Friedrich-Schiller-Univ. Jena (Germany)
Michael Kiehntopf, Friedrich-Schiller-Univ. Jena (Germany)
Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany)
Institute of Photonic Technology (IPHT) (Germany)
Petra Rösch, Friedrich-Schiller-Univ. Jena (Germany)
Michael Kiehntopf, Friedrich-Schiller-Univ. Jena (Germany)
Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany)
Institute of Photonic Technology (IPHT) (Germany)
Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)
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