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Proceedings Paper

Calibration phantom for the quantification of fluorescent labels in deep skin tissue
Author(s): Sebastian Beer; Ulf Maeder; Thorsten Bergmann; Peggy Schlupp; Thomas Schmidts; Johannes T. Heverhagen; Frank Runkel; Martin Fiebich
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Paper Abstract

A method to quantify fluorescent labels spatially resolved in scattering and absorbing samples is proposed and tested using a tissue phantom. The method works without any a priori knowledge about the optical properties of the sample. The scattering and absorption behavior of the sample is estimated by measuring reflectance from the sample simultaneously to the fluorescence. With this estimation, the attenuation of the fluorescence caused by scattering and absorption can be mathematically compensated. The method is planned to be used for evaluating skin penetrating drug carrier systems.

Paper Details

Date Published: 8 June 2011
PDF: 4 pages
Proc. SPIE 8086, Advanced Microscopy Techniques II, 80861L (8 June 2011); doi: 10.1117/12.889973
Show Author Affiliations
Sebastian Beer, Technische Hochschule Mittelhessen (Germany)
Ulf Maeder, Technische Hochschule Mittelhessen (Germany)
Thorsten Bergmann, Technische Hochschule Mittelhessen (Germany)
Peggy Schlupp, Technische Hochschule Mittelhessen (Germany)
Thomas Schmidts, Technische Hochschule Mittelhessen (Germany)
Johannes T. Heverhagen, Philipps Univ. Marburg (Germany)
Frank Runkel, Technische Hochschule Mittelhessen (Germany)
Martin Fiebich, Technische Hochschule Mittelhessen (Germany)

Published in SPIE Proceedings Vol. 8086:
Advanced Microscopy Techniques II
Peter T. C. So; Emmanuel Beaurepaire, Editor(s)

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