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Proceedings Paper

In vitro analysis of low-level laser irradiation on human osteoblast-like cells proliferation
Author(s): Nora Bloise; Enrica Saino; Francesca Bragheri; Paolo Minzioni; Ilaria Cristiani; Marcello Imbriani; Livia Visai
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Paper Abstract

The objective of this study was to examine the in vitro effect of a single or a multiple doses of low-level laser irradiation (LLLI) on proliferation of the human osteosarcoma cell line, SAOS-2. SAOS-2 cells were divided in five groups and exposed to LLLI (659 nm diode laser; 11 mW power output): group I as a control (dark), group II exposed to a single laser dose of 1 J/cm2, group III irradiated with a single dose of 3 J/cm2, and group IV and V exposed for three consecutive days to 1 or 3 J/cm², respectively. Cellular proliferation was assessed daily up to 7 days of culturing. The obtained results showed an increase in proliferative capacity of SAOS-2 cells during the first 96 h of culturing time in once-irradiated cells, as compared to control cells. Furthermore, a significantly higher proliferation in the group IV and V was detected if compared to a single dose or to control group after 96 h and 7 days. In conclusion, the effect of the single dose on cell proliferation was transitory and repeated irradiations were necessary to observe a strong enhancement of SAOS-2 growth. As a future perspective, we would like to determine the potential of LLLI as a new approach for promoting bone regeneration onto biomaterials.

Paper Details

Date Published: 2 June 2011
PDF: 5 pages
Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 80920U (2 June 2011); doi: 10.1117/12.889873
Show Author Affiliations
Nora Bloise, Univ. of Pavia (Italy)
Enrica Saino, Univ. of Pavia (Italy)
Francesca Bragheri, Univ. of Pavia (Italy)
Paolo Minzioni, Univ. of Pavia (Italy)
Ilaria Cristiani, Univ. of Pavia (Italy)
Marcello Imbriani, Univ. of Pavia (Italy)
Salvatore Maugeri Foundation (Italy)
Livia Visai, Univ. of Pavia (Italy)
Salvatore Maugeri Foundation (Italy)
International Ctr. for Studies and Research in Biomedicine (Luxembourg)

Published in SPIE Proceedings Vol. 8092:
Medical Laser Applications and Laser-Tissue Interactions V
Ronald Sroka; Lothar D. Lilge, Editor(s)

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