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Proceedings Paper

Image analysis of free diffusing nanoparticles in vitro
Author(s): Thorsten Wagner; Sven O. Luettmann; Dominic Swarat; Martin Wiemann; Hans-Gerd Lipinski
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Paper Abstract

Based on laser technology it is possible to visualize nanoparticles (NP) as light scattering objects by means of a conventional light microscope. Image series of visualized NPs were used to calculate characteristic parameters such as particle size and diffusion coefficient. Here progressive agglomeration of NP upon changes of ionic strength could be followed by repeated observation over minutes. While these analyses were based on dynamic properties of NPs, single particle paths were used to analyse particle agglomeration in more detail using image analysis methods. Together these techniques may help to better describe the behaviour of nanoparticles when used for cell culture experiments.

Paper Details

Date Published: 14 June 2011
PDF: 8 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808726 (14 June 2011); doi: 10.1117/12.889620
Show Author Affiliations
Thorsten Wagner, FH Dortmund (Germany)
Sven O. Luettmann, FH Dortmund (Germany)
Dominic Swarat, FH Dortmund (Germany)
Martin Wiemann, IBE RD gGmbH (Germany)
Hans-Gerd Lipinski, FH Dortmund (Germany)

Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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