Share Email Print

Proceedings Paper

Improved OCT imaging of lung tissue using a prototype for total liquid ventilation
Author(s): Christian Schnabel; Sven Meissner; Edmund Koch
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Optical coherence tomography (OCT) is used for imaging subpleural alveoli in animal models to gain information about dynamic and morphological changes of lung tissue during mechanical ventilation. The quality of OCT images can be increased if the refraction index inside the alveoli is matched to the one of tissue via liquid-filling. Thereby, scattering loss can be decreased and higher penetration depth and tissue contrast can be achieved. Until now, images of liquid-filled lungs were acquired in isolated and fixated lungs only, so that an in vivo measurement situation is not present. To use the advantages of liquid-filling for in vivo imaging of small rodent lungs, it was necessary to develop a liquid ventilator. Perfluorodecalin, a perfluorocarbon, was selected as breathing fluid because of its refraction index being similar to the one of water and the high transport capacity for carbon dioxide and oxygen. The setup is characterized by two independent syringe pumps to insert and withdraw the fluid into and from the lung and a custom-made control program for volume- or pressure-controlled ventilation modes. The presented results demonstrate the liquid-filling verified by optical coherence tomography and intravital microscopy (IVM) and the advantages of liquid-filling to OCT imaging of subpleural alveoli.

Paper Details

Date Published: 1 June 2011
PDF: 6 pages
Proc. SPIE 8091, Optical Coherence Tomography and Coherence Techniques V, 809116 (1 June 2011); doi: 10.1117/12.889463
Show Author Affiliations
Christian Schnabel, Dresden Univ. of Technology (Germany)
Sven Meissner, Dresden Univ. of Technology (Germany)
Edmund Koch, Dresden Univ. of Technology (Germany)

Published in SPIE Proceedings Vol. 8091:
Optical Coherence Tomography and Coherence Techniques V
Rainer A. Leitgeb; Brett E. Bouma, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?