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Proceedings Paper

Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe
Author(s): Rajesh S. Pillai; Dirk Lorenser; David D. Sampson
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Paper Abstract

We report on the design and implementation of a 350 μm-diameter confocal imaging probe based on gradient-index (GRIN) optics and a fiber-based scanning arrangement. The form factor of the probe is such that it can potentially be inserted into a 22-gauge hypodermic needle to perform high-resolution confocal fluorescence imaging in solid tissues. We introduce a simple scanning arrangement based on lensed fiber, which eliminates off-axis aberrations induced by conventional scanning optics and is suitable for integration into a compact hand-held unit. We present the details of the optical design and experimental verification of the performance of the optical system. The measured lateral resolution of ~700 nm is in agreement with the optical design and is the highest resolution reported for a confocal fluorescence imaging probe of this size. Further, we demonstrate the imaging capability of the probe by obtaining high-resolution images of fluorescently labeled muscle fibers.

Paper Details

Date Published: 17 May 2011
PDF: 4 pages
Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77534M (17 May 2011); doi: 10.1117/12.887373
Show Author Affiliations
Rajesh S. Pillai, The Univ. of Western Australia (Australia)
Dirk Lorenser, The Univ. of Western Australia (Australia)
David D. Sampson, The Univ. of Western Australia (Australia)

Published in SPIE Proceedings Vol. 7753:
21st International Conference on Optical Fiber Sensors
Jacques Albert; Wojtek J. Bock, Editor(s)

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