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Proceedings Paper

Raman spectroscopy of HIV-1 antigen and antibody
Author(s): Pavel V. Zinin; Ningjie Hu; Lori E. Kamemoto; Qigui Yu; Anupam K. Misra; Shiv K. Sharma
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Paper Abstract

Raman spectra of anti-HIV-1 antibody, HIV-1 antigen (p24), and HIV-1 antibody-antigen complex have been measured in near-infrared and UV regions: 785 nm; 830 nm; and 244 nm laser excitations. The spectrum of the HIV-1 antigen was excited with an infrared laser and contains numerous Raman peaks. The most prominent peaks are broad bands at 1343, 1449, 1609 and 1655 cm-1, which are characteristic of the Raman spectra of biological cells. The UV Raman spectrum of the HIV-1 antigen has a completely different structure. It has two strong peaks at 1613 cm-1 and 1173 cm-1. The peak at 1613 cm-1 is associated with vibrations of the aromatic amino acids tyrosine (Tyr) and tryptophan (Try). The second strongest peak at 1173 cm-1 is associated with the vibration of Tyr. The Raman peak pattern of the HIV-1 antigen-antibody complex is very similar to that of the HIV-1 antigen. The only difference is that the peak at 1007 cm-1 of the Raman spectrum of the HIV-1 antigen-antibody complex is slightly enhanced compared to that of the HIV-1 antigen. This indicates that the peaks of the HIV-1 antigen dominate the Raman spectrum of the HIV-1 antigen-antibody complex.

Paper Details

Date Published: 26 May 2011
PDF: 7 pages
Proc. SPIE 8025, Smart Biomedical and Physiological Sensor Technology VIII, 80250D (26 May 2011); doi: 10.1117/12.887169
Show Author Affiliations
Pavel V. Zinin, Univ. of Hawai'i (United States)
Ningjie Hu, Indiana Univ. School of Medicine (United States)
Lori E. Kamemoto, John A. Burns School of Medicine, Univ. of Hawai'i (United States)
Qigui Yu, Indiana Univ. School of Medicine (United States)
Anupam K. Misra, Univ. of Hawai'i (United States)
Shiv K. Sharma, Univ. of Hawai'i (United States)


Published in SPIE Proceedings Vol. 8025:
Smart Biomedical and Physiological Sensor Technology VIII
Brian M. Cullum; Eric S. McLamore, Editor(s)

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