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Proceedings Paper

Integration of optical devices and nanotechnology for conducting genome research
Author(s): Pei-Yu Chung; Parekh Parag; Zhi Zhu; Claudine Chegini; Gregory Schultz; Weihong Tan; Peng Jiang; Christopher Batich
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Paper Abstract

SPR based sensing techniques utilize a spectroscopy for transducing biomolecular binding events to variations in spectra. This label-free and real-time technique has widely applied for conducting biomedical research. In this study, we present a spectroscopy-based SPR system for monitoring binding between human serum albumin and nucleic acid library. Compared with conventional SPR technique, this novel system utilizes cost-effective nanostructured arrays and a portable UV-Vis spectrometer. These advantages enable a promising development of a portable analytical device for widespread applications. Meanwhile, multispectral analysis used here also helps increase the sensitivity, and thus transducing the binding event to optical signal efficiently. The result demonstrates that this cost-effective and portable system could be applied for a future application of selecting target aptamer. Moreover, we also present surface enhanced Raman spectroscopy (SERS) on the nanostructured arrays in a label-free approach. This integration of multiple spectroscopy technologies is utilized for conducting genome research efficiently.

Paper Details

Date Published: 12 May 2011
PDF: 7 pages
Proc. SPIE 8032, Next-Generation Spectroscopic Technologies IV, 80320J (12 May 2011); doi: 10.1117/12.887154
Show Author Affiliations
Pei-Yu Chung, Univ. of Florida (United States)
Parekh Parag, Univ. of Florida (United States)
Zhi Zhu, Univ. of Florida (United States)
Claudine Chegini, Univ. of Florida (United States)
Gregory Schultz, Univ. of Florida (United States)
Weihong Tan, Univ. of Florida (United States)
Peng Jiang, Univ. of Florida (United States)
Christopher Batich, Univ. of Florida (United States)

Published in SPIE Proceedings Vol. 8032:
Next-Generation Spectroscopic Technologies IV
Mark A. Druy; Richard A. Crocombe, Editor(s)

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