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Proceedings Paper

An optical biosensor based on His6-OPH for organophosphate detection
Author(s): Nina Frančič; Ilya V. Lyagini; Elena N. Efremenko; Aljoša Košak; Aleksandra Lobnik
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Paper Abstract

In this work we describe a method for immobilizing the hexahistidine-modified OPH (His6-OPH) enzyme via sol-gel technology in a porous material while retaining its catalytic activity. The potential use of each bio-sensing material was checked by quantifying the enzymatic properties, such as the relative activity of the immobilized enzyme, its Michaelis- Menten equation parameters and its stability under extreme working conditions (pH, T). The bio-sensor material was also characterised by SEM as well as in terms of its reversibility and sensitivity.

Paper Details

Date Published: 6 May 2011
PDF: 7 pages
Proc. SPIE 8073, Optical Sensors 2011; and Photonic Crystal Fibers V, 80730T (6 May 2011); doi: 10.1117/12.886788
Show Author Affiliations
Nina Frančič, Univ. of Maribor (Slovenia)
Ilya V. Lyagini, M. V. Lomonosov Moscow State Univ. (Russian Federation)
Elena N. Efremenko, M. V. Lomonosov Moscow State Univ. (Russian Federation)
Aljoša Košak, Univ. of Maribor (Slovenia)
Aleksandra Lobnik, Univ. of Maribor (Slovenia)

Published in SPIE Proceedings Vol. 8073:
Optical Sensors 2011; and Photonic Crystal Fibers V
Kyriacos Kalli; Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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