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Proceedings Paper

Haemoglobin-Triton X-100 conjugate as model system for red blood cell lysis
Author(s): Simona-Florentina Pop; Rodica-Mariana Ion; Sanda Doncea
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Paper Abstract

The action of detergents is thought to be connected primarily with micelle formation. However, detergent monomers can also affect biological systems. It was found that human red blood cells can be disintegrated with Triton X-100 non-ionic detergent at a concentration of 0.007%, lower than the critical micellar concentration (CMC). The lytic membrane of non-ionic detergent Triton X-100 (as a model), and its ability to lyse red blood cells in vitro used as an indicator of conjugate conformation at different pHs. The time dependent release of hemoglobin (Hb) and potassium from red blood cells was detected at 37 °C and both were sigmoid in character. Although Triton X-100 was highly lytic at pH 5.5, 7.4 and 8.0, the conjugate only show a lysis concentration-dependent of red blood cell at pH 5.5. Triton X-100 causes the Hb to aggregate, a condition that can be simulated when this non-ionic surfactant is incubated with Hb in vitro. The determination of Triton-X was done by HPLC, in accordance to characterize the surfactant. The increased stability in micellar medium can be attributed to deep penetration with the polar group -OH oriented towarded to the micelle surface. Thermal stability of hemoglobin has been investigated in order to evaluate the nature of thermal behavior of this compound. We studied the effects of surfactant Triton -X on the rate constants for the destroying of hemoglobin.

Paper Details

Date Published: 3 December 2010
PDF: 6 pages
Proc. SPIE 7821, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies V, 78210L (3 December 2010); doi: 10.1117/12.882150
Show Author Affiliations
Simona-Florentina Pop, ICECHIM (Romania)
Valahia Univ. of Targoviste (Romania)
Rodica-Mariana Ion, ICECHIM (Romania)
Valahia Univ. of Targoviste (Romania)
Sanda Doncea, ICECHIM (Romania)
Valahia Univ. of Targoviste (Romania)

Published in SPIE Proceedings Vol. 7821:
Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies V
Paul Schiopu; George Caruntu, Editor(s)

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