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Proceedings Paper

Development of a hybrid MRI and fluorescence tomography system for small animal imaging
Author(s): Michael T. Ghijsen; Yuting Lin; Orhan Nalcioglu; Gultekin Gulsen
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Paper Abstract

Fluorescence diffuse optical tomography (FT) is a molecular imaging technique that can create images of spatially resolved fluorophore concentrations and fluorescence lifetimes. One problem faced by FT is that the recovered fluorophore parameters greatly depend on the size and depth of the inclusion due to the ill-posedness of the FT inverse problem. Structural a priori information from imaging modalities with high spatial resolution is demonstrated to significantly improve the accuracy of the FT reconstruction. We have constructed a hybrid FT/MRI system for small animal imaging in this study. Near-infrared light was delivered and collected by optical fibers that connect the FT/MRI system to the interface in the MRI bore. We investigated the feasibility of a photo-multiplier tube (PMT) based detection system that acquired time-resolved data in the frequency domain. Phantom studies were used to evaluate the performance of the combined system. The concentration and lifetime maps were reconstructed with and without the structural a priori information obtained from MRI. ICG and DTTCI, two fluorophores with similar excitation and emission spectra but different lifetimes, were used in this evaluation. Specifically, we showed that the PMT-based frequency domain hybrid system was capable of differentiating between two fluorophores with different fluorescence lifetimes. Furthermore, this process was shown to be more accurate when MR a priori is used.

Paper Details

Date Published: 21 February 2011
PDF: 5 pages
Proc. SPIE 7892, Multimodal Biomedical Imaging VI, 789212 (21 February 2011); doi: 10.1117/12.881166
Show Author Affiliations
Michael T. Ghijsen, Univ. of California, Irvine (United States)
Yuting Lin, Univ. of California, Irvine (United States)
Orhan Nalcioglu, Univ. of California, Irvine (United States)
Gultekin Gulsen, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 7892:
Multimodal Biomedical Imaging VI
Fred S. Azar; Xavier Intes, Editor(s)

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