The wild-type phototropin protein phot from the green alga Chlamydomonas reinhardtii consists of two N-terminal LOV domains LOV1 and LOV2 with flavin mononucleotide (FMN) cofactor and a C-terminal serine-threonine kinase domain. It controls multiple steps in the sexual lifecycle of the alga. Here the LOV1-His domain of phot with modified cofactor is studied. FMN is replaced by roseoflavin monophosphate (8-dimethylamino-8-demethyl-FMN, RoFMN). The modified LOV1 domain is called RoLOV1. The photo-dynamics consequences of the cofactor change are studied. The absorption, emission, and photo-cyclic behaviour of LOV1-His and RoLOV1-His are compared. A spectroscopic characterisation of the cofactors FMN and RoFMN (roseoflavin) is given.

Date Published: 11 January 2011
PDF: 10 pages
Proc. SPIE 7993, ICONO 2010: International Conference on Coherent and Nonlinear Optics, 79931E (11 January 2011); doi: 10.1117/12.879134

Published in SPIE Proceedings Vol. 7993:
ICONO 2010: International Conference on Coherent and Nonlinear Optics
Claude Fabre; Victor Zadkov; Konstantin Drabovich, Editor(s)