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Proceedings Paper

Photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor
Author(s): A. Tyagi; A. Penzkofer; T. Mathes; P. Hegemann
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Paper Abstract

The wild-type phototropin protein phot from the green alga Chlamydomonas reinhardtii consists of two N-terminal LOV domains LOV1 and LOV2 with flavin mononucleotide (FMN) cofactor and a C-terminal serine-threonine kinase domain. It controls multiple steps in the sexual lifecycle of the alga. Here the LOV1-His domain of phot with modified cofactor is studied. FMN is replaced by roseoflavin monophosphate (8-dimethylamino-8-demethyl-FMN, RoFMN). The modified LOV1 domain is called RoLOV1. The photo-dynamics consequences of the cofactor change are studied. The absorption, emission, and photo-cyclic behaviour of LOV1-His and RoLOV1-His are compared. A spectroscopic characterisation of the cofactors FMN and RoFMN (roseoflavin) is given.

Paper Details

Date Published: 11 January 2011
PDF: 10 pages
Proc. SPIE 7993, ICONO 2010: International Conference on Coherent and Nonlinear Optics, 79931E (11 January 2011); doi: 10.1117/12.879134
Show Author Affiliations
A. Tyagi, Univ. Regensburg (Germany)
A. Penzkofer, Univ. Regensburg (Germany)
T. Mathes, Humboldt Univ. zu Berlin (Germany)
P. Hegemann, Humboldt Univ. zu Berlin (Germany)

Published in SPIE Proceedings Vol. 7993:
ICONO 2010: International Conference on Coherent and Nonlinear Optics
Claude Fabre; Victor Zadkov; Konstantin Drabovich, Editor(s)

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