
Proceedings Paper
Tyrosinase-catalyzed melanin as a contrast agent for photoacoustic tomographyFormat | Member Price | Non-Member Price |
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Paper Abstract
It is difficult to distinguish between tumor cells and surrounding cells without staining as is done in histology. We
developed tyrosinase-catalyzed melanin as a reporter gene for photoacoustic tomography. Tyrosinase is the primary
enzyme responsible for the production of melanin and alone is sufficient to produce melanin in non-melanogenic cells.
Two cell lines were created: a stably transfected HeLa line and a transiently transfected 293 line. A phantom
experiment was performed with the 293 transfected cells 48 hours post transfection and the results compared with
oxygenated whole blood, B16 melanoma and 293 control cells. An in vivo experiment was performed using the
transfected HeLa cells xenografted into a nude mouse ear, and then imaged. The results show strong contrast for
tyrosinase-catalyzed melanin in both the 293 cells in the tube phantom as well as the in vivo result showing melanin in
a nude mouse ear. Transfection increased expression in 293 cells 159 fold and image contrast compared to blood by as
much as 50 fold. Due to the strong signal obtained at longer wavelengths and the decrease of blood signal at the same
wavelengths, tyrosinase catalyzed melanin is a good candidate as a molecular imaging contrast agent for photoacoustic
tomography.
Paper Details
Date Published: 17 February 2011
PDF: 6 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78991G (17 February 2011); doi: 10.1117/12.879110
Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)
PDF: 6 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78991G (17 February 2011); doi: 10.1117/12.879110
Show Author Affiliations
Arie Krumholz, Washington Univ. in St. Louis (United States)
Sarah Chavez, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Sarah Chavez, Washington Univ. in St. Louis (United States)
Junjie Yao, Washington Univ. in St. Louis (United States)
Timothy Fleming, Washington Univ. in St. Louis (United States)
William E. Gillanders, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
William E. Gillanders, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)
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