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Proceedings Paper

Photothermal therapy of acute leukemia cells in the near-infrared region using gold nanorods CD-33 conjugates
Author(s): Anton Liopo; André Conjusteau; Marina Konopleva; Michael Andreeff; Alexander Oraevsky
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Paper Abstract

In the present work, we demonstrate a potential use of gold nanorods as a contrast agent for selective photothermal therapy of human acute leukemia cells (HL-60) using a near-infrared laser. Gold Nanorods (GNR) are synthesized and conjugated to CD33, a 67 kDa glycoprotein found on the surface of myeloid cells that belongs to the sialoadhesin family of proteins. After pegylation, or conjugation with CD33 antibody, GNR were non-toxic for acute and chronic leukemia cells. We used a Quanta System q-switched titanium sapphire laser emitting at a center wavelength of 755 nm. Each sample was illuminated with 1 laser shot at either high or low fluence. Both laser modes were used in 3 independent cell probes. HL-60 cells were treated for 45 min with GNR conjugated with mAb CD33, or with GNR-Pegylated particles. After laser application, the cells were resuspended and analyzed to cell viability with Trypan blue exclusion assay. GNR-CD33 conjugates significantly increase the percentage of cell death as compared with a control group after laser illumination: a 3 fold increase is observed.

Paper Details

Date Published: 28 February 2011
PDF: 8 pages
Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 789710 (28 February 2011); doi: 10.1117/12.878802
Show Author Affiliations
Anton Liopo, TomoWave Labs., Inc. (United States)
André Conjusteau, TomoWave Labs., Inc. (United States)
Marina Konopleva, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Michael Andreeff, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Alexander Oraevsky, TomoWave Labs., Inc. (United States)

Published in SPIE Proceedings Vol. 7897:
Optical Interactions with Tissue and Cells XXII
E. Duco Jansen; Robert J. Thomas, Editor(s)

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