
Proceedings Paper
Characterization of third-degree burned skin by nonlinear microscopy techniqueFormat | Member Price | Non-Member Price |
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Paper Abstract
Nonlinear microscopy imaging technique enable take both images of collagen fibers in dermis through second harmonic
generation (SHG) signal and elastic fibers by two-photon emission fluorescence microscopy (TPEFM). These techniques
are the most commonly used technique for turbid and thick tissue imaging and also to image biological samples which
presents highly ordered structural proteins without any exogenous label. The objective of this study is characterizing
dermis of third-degree burned skin by TPEFM and SHG technique. The modelocked laser (Spectra Physics) source used
in this study with pulse width of approximately 100 fs at 80 MHz was directed into a multiphoton microscope using a
laser scanning unit (Olympus Fluoview 300), mounted on an inverted confocal system microscope (Olympus IX81), with
focusing objective (40x, NA = 1.30). The samples were obtained from Wistar rats, male, adult. One dorsum area was
submitted to burn caused by vapour exposure. The biopsies obtained were cryosectioned in slices of 20 μm width.
Selected area of interface between the injured and healthy subdermal burned skin were imaged by TPEFM and SHG
technique. Two different autofluorescence signals are observed as a function of excitation wavelength. The
autofluorescence observed at 760 nm and 690 nm suggest components of extracellular matrix at differents depths. In
SHG images, collagen fibers are visible. According to the images obtained, these methodologies can be used to
characterize dermis of burned tissue as its healing process with reduced out-of-plane photobleaching and phototoxicity.
Paper Details
Date Published: 11 February 2011
PDF: 5 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032Y (11 February 2011); doi: 10.1117/12.878763
Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)
PDF: 5 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032Y (11 February 2011); doi: 10.1117/12.878763
Show Author Affiliations
Moisés Oliveira dos Santos, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Vitor Bianchini Pelegati, Univ. Estadual de Campinas (Brazil)
Carlos Lenz Cesar, Univ. Estadual de Campinas (Brazil)
Vitor Bianchini Pelegati, Univ. Estadual de Campinas (Brazil)
Carlos Lenz Cesar, Univ. Estadual de Campinas (Brazil)
Paulo Roberto Correa, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Telma Maria Tenório Zorn, Univ. de São Paulo (Brazil)
Denise Maria Zezell, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Telma Maria Tenório Zorn, Univ. de São Paulo (Brazil)
Denise Maria Zezell, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)
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