
Proceedings Paper
3D visualization and quantification of bone and teeth mineralization for the study of osteo/dentinogenesis in mice modelsFormat | Member Price | Non-Member Price |
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Paper Abstract
Research on bone and teeth mineralization in animal models is critical for understanding human pathologies. Genetically
modified mice represent highly valuable models for the study of osteo/dentinogenesis defects and osteoporosis. Current
investigations on mice dental and skeletal phenotype use destructive and time consuming methods such as histology and
scanning microscopy. Micro-CT imaging is quicker and provides high resolution qualitative phenotypic description.
However reliable quantification of mineralization processes in mouse bone and teeth are still lacking. We have
established novel CT imaging-based software for accurate qualitative and quantitative analysis of mouse mandibular
bone and molars.
Data were obtained from mandibles of mice lacking the Fibromodulin gene which is involved in mineralization
processes. Mandibles were imaged with a micro-CT originally devoted to industrial applications (Viscom, X8060 NDT).
3D advanced visualization was performed using the VoxBox software (UsefulProgress) with ray casting algorithms.
Comparison between control and defective mice mandibles was made by applying the same transfer function for each 3D
data, thus allowing to detect shape, colour and density discrepencies. The 2D images of transverse slices of mandible and
teeth were similar and even more accurate than those obtained with scanning electron microscopy. Image processing of
the molars allowed the 3D reconstruction of the pulp chamber, providing a unique tool for the quantitative evaluation of
dentinogenesis.
This new method is highly powerful for the study of oro-facial mineralizations defects in mice models,
complementary and even competitive to current histological and scanning microscopy appoaches.
Paper Details
Date Published: 9 March 2011
PDF: 6 pages
Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79650J (9 March 2011); doi: 10.1117/12.878251
Published in SPIE Proceedings Vol. 7965:
Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging
John B. Weaver; Robert C. Molthen, Editor(s)
PDF: 6 pages
Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79650J (9 March 2011); doi: 10.1117/12.878251
Show Author Affiliations
A. Marchadier, Institut PRISME (France)
C. Vidal, Institut Pasteur, INSERM (France)
S. Ordureau, UsefulProgress (France)
R. Lédée, Institut PRISME (France)
C. Vidal, Institut Pasteur, INSERM (France)
S. Ordureau, UsefulProgress (France)
R. Lédée, Institut PRISME (France)
C. Léger, Institut PRISME (France)
M. Young, National Institutes of Health (United States)
M. Goldberg, Institut Pasteur, INSERM (France)
M. Young, National Institutes of Health (United States)
M. Goldberg, Institut Pasteur, INSERM (France)
Published in SPIE Proceedings Vol. 7965:
Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging
John B. Weaver; Robert C. Molthen, Editor(s)
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