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Proceedings Paper

Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles
Author(s): Jae Youn Hwang; Zeev Gross; Harry B. Gray; Lali K. Medina-Kauwe; Daniel L. Farkas
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Paper Abstract

We report the feasibility of tumor detection and delineation in vivo using multimode optical imaging of targeted gallium corrole (HerGa). HerGa is highly effective for targeted HER2+ tumor elimination in vivo, and it emits intense fluorescence. These unique characteristics of HerGa prompted us to investigate the potential of HerGa for tumor detection and delineation, by performing multimode optical imaging ex vivo and in vivo; the imaging modes included fluorescence intensity, spectral (including ratiometric), lifetime, and two-photon excited fluorescence, using our custombuilt imaging system. While fluorescence intensity imaging provided information about tumor targeting capacity and tumor retention of HerGa, ratiometric spectral imaging offered more quantitative and specific information about HerGa location and accumulation. Most importantly, the fluorescence lifetime imaging of HerGa allowed us to discriminate between tumor and non-tumor regions by fluorescence lifetime differences. Finally, two-photon excited fluorescence images provided highly resolved and thus topologically detailed information around the tumor regions where HerGa accumulates. Taken together, the results shown in this report suggest the feasibility of tumor detection and delineation by multimode optical imaging of HerGa, and fluorescent chemotherapy agents in general. Specifically, the multimode optical imaging can offer complementary and even synergetic information simultaneously in the tumor detection and delineation by HerGa, thus enhancing contrast.

Paper Details

Date Published: 28 February 2011
PDF: 8 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020F (28 February 2011); doi: 10.1117/12.877780
Show Author Affiliations
Jae Youn Hwang, Cedars-Sinai Medical Ctr. (United States)
Univ. of Southern California, Los Angeles (United States)
Zeev Gross, Beckman Research Institute (United States)
Technion-Israel Institute of Technology (Israel)
Harry B. Gray, Beckman Research Institute (United States)
Lali K. Medina-Kauwe, Cedars-Sinai Medical Ctr. (United States)
Univ. of California, Los Angeles (United States)
Daniel L. Farkas, Univ. of Southern California (United States)
Spectral Molecular Imaging, Inc. (United States)

Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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