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Proceedings Paper

Toward an on-chip multiplexed nucleic acid hybridization assay using immobilized quantum dot-oligonucleotide conjugates and fluorescence resonance energy transfer
Author(s): Anthony J. Tavares; M. Omair Noor; W. Russ Algar; Charles H. Vannoy; Lu Chen; Ulrich J. Krull
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Paper Abstract

Semiconductor quantum dots (QD) are a class of NP with photophysical properties that are ideally suited for optical multiplexing and use as donors in fluorescence resonance energy transfer (FRET). A new strategy is presented for the development of multiplexed DNA hybridization assays using immobilized QDs in a microfluidic system. Green- or red-emitting QDs were immobilized via self-assembly with a multidentate-thiol-derivatized glass slide, and subsequently conjugated with amine-terminated probe oligonucleotides using carbodiimide activation. Immobilized QD-probe conjugates were then passivated with adsorbed non-complementary oligonucleotides to achieve selectivity in microfluidic assays. Target nucleic acid sequences hybridized with QD-probe conjugates and were labeled with Cy3 or Alexa Fluor 647 as acceptor dyes for the QD donors, where FRET-sensitized dye emission provided a signal for the detection of picomolar quantities of target. The simultaneous immobilization of green- and red-emitting QDs at different ratios within a microfluidic channel was demonstrated as a step toward multiplexed assays.

Paper Details

Date Published: 11 February 2011
PDF: 11 pages
Proc. SPIE 7909, Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI, 79090X (11 February 2011); doi: 10.1117/12.877326
Show Author Affiliations
Anthony J. Tavares, Univ. of Toronto Mississauga (Canada)
M. Omair Noor, Univ. of Toronto Mississauga (Canada)
W. Russ Algar, Univ. of Toronto Mississauga (Canada)
Charles H. Vannoy, Univ. of Toronto Mississauga (Canada)
Lu Chen, Univ. of Toronto Mississauga (Canada)
Ulrich J. Krull, Univ. of Toronto Mississauga (Canada)

Published in SPIE Proceedings Vol. 7909:
Colloidal Quantum Dots/Nanocrystals for Biomedical Applications VI
Wolfgang J. Parak; Kenji Yamamoto M.D.; Marek Osinski, Editor(s)

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