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Proceedings Paper

Taxol-induced paraptosis-like A549 cell death is not senescence
Author(s): Chao-yang Wang; Tong-Sheng Chen
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Paper Abstract

Our previous studies have shown that taxol, a potent anticancer agent, induces caspase-independent cell death and cytoplasmic vacuolization in human lung cancer cells. However, the mechanisms of taxol-induced cytoplasmic vacuolization are poorly understood. Cytoplasmic vacuolization have been reported to be a characteristic of cell senescence. Here, we employed confocal fluorescence microscopy imaging to study the reversibility of taxol-induced cytoplasmic vacuolization and whether taxol triggers senescence in A549 cells. We found that taxol-induced cytoplasmic vacuolization at 6 or 9 h after treatment with taxol did not decrease but increase at 24 h or 72 h after refreshing the culture medium without taxol, indicating taxol-induced cytoplasmic vacuolization is irreversible. We used SA-β-Gal (senescence-associated β-galactosidase) to assess whether taxol-induced cell death in cytoplasmic vacuolization fashion is senescence, and found that hydrogen peroxide (H2O2)-treated, but not taxol-treated cells is significantly stained by the SA-β-Gal, a senescence testing kit, indicating that the form of taxol-induced cell death is not senescence.

Paper Details

Date Published: 10 February 2011
PDF: 6 pages
Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000K (10 February 2011); doi: 10.1117/12.877056
Show Author Affiliations
Chao-yang Wang, South China Normal Univ. (China)
Tong-Sheng Chen, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 7900:
Biophotonics and Immune Responses VI
Wei R. Chen, Editor(s)

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