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Proceedings Paper

Rapid analysis of white blood cells with diffraction imaging flow cytometry
Author(s): Xin-Hua Hu; Jun Q. Lu; Yuanming Feng
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Paper Abstract

From a foundation of cell optics research program we have recently developed a diffraction imaging flow cytometer method which can be used to rapidly acquire and analyze diffraction image data from single flowing cells. Modeling and experimental investigation of light scattering by white blood cells (WBC) have been conducted which suggest a strong correlation between the fringe patterns related to the texture of the diffraction images and 3D morphological features of the cells. In particular, we present results of calculated and measured diffraction images obtained with three cell lines derived from leukemia patients to demonstrate that texture features of the diffraction images extracted with a gray-scale co-occurrence-matrix algorithm can be used for rapid cell classification.

Paper Details

Date Published: 11 February 2011
PDF: 7 pages
Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070W (11 February 2011); doi: 10.1117/12.876702
Show Author Affiliations
Xin-Hua Hu, East Carolina Univ. (United States)
Jun Q. Lu, East Carolina Univ. (United States)
Yuanming Feng, Tianjin Univ. (China)

Published in SPIE Proceedings Vol. 7907:
Biomedical Applications of Light Scattering V
Adam P. Wax; Vadim Backman, Editor(s)

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