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Proceedings Paper

Adaptive optics in wide-field microscopy
Author(s): Peter Kner; Zvi Kam; David Agard; John Sedat
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Paper Abstract

Three-dimensional live imaging in cell biology is hindered by optical aberrations which degrade the resolution and signal-to-noise ratio as the focal plane is moved deeper into the sample. The solution to this problem is to use adaptive optics to correct the aberrations. In this paper, we discuss our work on applying adaptive optics to wide-field fluorescence microscopy. We demonstrate correction of depth-aberrations and focusing using a deformable mirror in open-loop operation. We then discuss the use of phase retrieval and phase diversity in adaptive optics.

Paper Details

Date Published: 14 February 2011
PDF: 12 pages
Proc. SPIE 7931, MEMS Adaptive Optics V, 79310K (14 February 2011); doi: 10.1117/12.876500
Show Author Affiliations
Peter Kner, The Univ. of Georgia (United States)
Zvi Kam, Weizmann Institute of Science (Israel)
David Agard, Univ. of California, San Francisco (United States)
John Sedat, Univ. of California, San Francisco (United States)

Published in SPIE Proceedings Vol. 7931:
MEMS Adaptive Optics V
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)

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