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Proceedings Paper

Robust detection of peak signals for lateral flow immunoassays
Author(s): Jongwon Kim; Jong Dae Kim; Kie Bong Nahm; Eui Yul Choi; Geumyoung Lee
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Paper Abstract

Template matching method is presented to identify the peaks from the scanned signals of lateral flow immunoassay strips. The template is composed of two pulses separated by the distance of the control and the target ligand line in the assay, and is convolved with the scanned signal to deliver the maximum at the center of the two peaks. The peak regions were identified with the predefined distances from the center. Glycosylated haemoglobin immunoassay strips and fluorescent strip readers from Boditechmed Inc. were tested to estimate the lot and reader variations of the concentration measurands. The results showed the robustness of the propose method.

Paper Details

Date Published: 14 February 2011
PDF: 7 pages
Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 792910 (14 February 2011); doi: 10.1117/12.876260
Show Author Affiliations
Jongwon Kim, Biomedlab Co., Ltd. (Korea, Republic of)
Jong Dae Kim, Hallym Univ. (Korea, Republic of)
Kie Bong Nahm, Hallym Univ. (Korea, Republic of)
Eui Yul Choi, Hallym Univ. (Korea, Republic of)
Geumyoung Lee, Biomedlab Co., Ltd. (Korea, Republic of)

Published in SPIE Proceedings Vol. 7929:
Microfluidics, BioMEMS, and Medical Microsystems IX
Holger Becker; Bonnie L. Gray, Editor(s)

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