
Proceedings Paper
In vivo multiscale photoacoustic microscopy of human skinFormat | Member Price | Non-Member Price |
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Paper Abstract
Scalability is a key feature of photoacoustic microscopy (PAM). Reports have shown that PAM systems can be
designed to possess sub-micron resolution at shallow depths or penetrate centimeters deep at the expense of
resolution while the number of resolved pixels in the depth direction remains high. This capability to readily tune
the imaging parameters while maintaining the same inherent contrast could be extremely useful for a variety of
biomedical applications. Human skin, with its layered vascular structure whose dimensions scale with depth,
provides an ideal imaging target to illustrate this advantage. Here, we present results from in vivo human skin
imaging experiments using two different PAM systems, an approach which enables better characterization of the
cutaneous microvasculature throughout the imaging depth. Specifically, we show images from several distinct areas
of skin: the palm and the forearm. For each region, the same area was imaged with both an optical-resolution PAM
(OR-PAM) and an acoustic-resolution PAM (AR-PAM), and the subsequent images were combined into composite
images. The OR-PAM provides less than 5 μm lateral resolution, capable of imaging the smallest capillary vessels,
while the AR-PAM enables imaging at depths of several millimeters. Several structures are identifiable in the ORPAM
images which cannot be differentiated in AR-PAM images, namely thin epidermal and stratum corneum
layers, undulations in the dermal papillae, and capillary loops. However, the AR-PAM provides images of larger
vessels, deeper than the OR-PAM can penetrate. These results demonstrate how PAM's scalability can be utilized to
more fully characterize cutaneous vasculature, potentially impacting the assessment of numerous cardiovascular
related and cutaneous diseases.
Paper Details
Date Published: 22 February 2011
PDF: 6 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789946 (22 February 2011); doi: 10.1117/12.875969
Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)
PDF: 6 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789946 (22 February 2011); doi: 10.1117/12.875969
Show Author Affiliations
Christopher P. Favazza, Washington Univ. in St. Louis (United States)
Song Hu, Washington Univ. in St. Louis (United States)
Victor Huang, Washington Univ. School of Medicine (United States)
Song Hu, Washington Univ. in St. Louis (United States)
Victor Huang, Washington Univ. School of Medicine (United States)
Omar Jassim, Washington Univ. School of Medicine (United States)
Lynn A. Cornelius, Washington Univ. School of Medicine (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Lynn A. Cornelius, Washington Univ. School of Medicine (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)
Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)
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