Share Email Print

Proceedings Paper

Local plasma membrane permeabilization of living cells by nanosecond electric pulses using atomic force microscopy
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Numerous studies provide evidence that nanosecond electric pulses (nsEPs) can trigger the formation of nanopores in the plasma membranes of cells. However, the biophysical mechanism responsible for nanopore formation is not well understood. In this study, we hypothesize that membrane damage induced by nsEPs is primarily dependent on the local molecular composition and mechanical strength of the plasma membrane. To test this hypothesis, we positioned metal-coated, nanoscale cantilever tips using an atomic force microscope (AFM) to deliver nsEPs to localized areas on the surface of the plasma membrane. We conducted computational modeling simulations to verify that the electric field provided by the nsEP is concentrated between the tip and the plasma membrane. The results show that we could effectively deliver nsEPs using the AFM tips at very low voltages. Using scanning electron microscopy we analyzed the tips after applying 10V over 5 seconds duration and found no damage to the tip or loss of platinum coating. As a proof of concept, we applied a 1 and 10V, 5 second pulse to HeLa cells causing large morphological changes. We also applied both a mechanical indention and 600ns electrical pulse stimulus and measured positive propidium ion uptake into the cytoplasm suggesting formation of membrane pores. In future studies, we plan to elucidate the effect that specific, local molecular structures and compositions have on efficacy of electroporation using the newly constructed nano-electrode system.

Paper Details

Date Published: 15 February 2011
PDF: 8 pages
Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080U (15 February 2011); doi: 10.1117/12.875711
Show Author Affiliations
Gary Thompson, Ball Aerospace Inc. (United States)
Clemson Univ. (United States)
Jason A. Payne, Air Force Research Lab. (United States)
Caleb C. Roth, General Dynamics Information Technology (United States)
Gerald J. Wilmink, Air Force Research Lab. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 7908:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

© SPIE. Terms of Use
Back to Top