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Proceedings Paper

3D myofibril imaging in live cardiomyocytes via hybrid SHG-TPEF microscopy
Author(s): Yonghong Shao; Honghai Liu; Tong Ye; Tom Borg; Junle Qu; Xiang Peng; Hanben Niu; Bruce Gao
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Paper Abstract

We developed a hybrid SHG-TPEF polarization imaging system that allowed the excitation beam from an fs Ti:Sappire laser being bi-directionally raster scanned across the focal plane using a pair of orthogonal galvanometers. To implement high-speed scanning, the turning regions of the triangular waves were smoothed by a custom-designed waveform. The SHG and TPEF signals from samples were recorded by two PMTs in the forward and backward direction. Using this imaging system, we obtained 3D images of the sarcomere structure via SHG and DiO-stained lipid membrane via TPEF in live cardiomyocytes isolated from neonatal and adult rats. The results demonstrated the potential applications of SHG and TPEF in the research of myofibrillogensis.

Paper Details

Date Published: 3 March 2011
PDF: 6 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030F (3 March 2011); doi: 10.1117/12.875607
Show Author Affiliations
Yonghong Shao, Shenzhen Univ. (China)
Honghai Liu, Clemson Univ. (United States)
Tong Ye, Univ. of Alabama at Birmingham (United States)
Tom Borg, Medical Univ. of South Carolina (United States)
Junle Qu, Shenzhen Univ. (China)
Xiang Peng, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)
Bruce Gao, Clemson Univ. (United States)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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