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Proceedings Paper

Adaptive optics two-photon scanning laser fluorescence microscopy
Author(s): Yaopeng Zhou; Thomas Bifano; Charles Lin
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Paper Abstract

Two-photon fluorescence microscopy provides a powerful tool for deep tissue imaging. However, optical aberrations from illumination beam path limit imaging depth and resolution. Adaptive Optics (AO) is found to be useful to compensate for optical aberrations and improve image resolution and contrast from two-photon excitation. We have developed an AO system relying on a MEMS Deformable Mirror (DM) to compensate the optical aberrations in a two-photon scanning laser fluorescence microscope. The AO system utilized a Zernike polynomial based stochastic parallel gradient descent (SPGD) algorithm to optimize the DM shape for wavefront correction. The developed microscope is applied for subsurface imaging of mouse bone marrow. It was demonstrated that AO allows 80% increase in fluorescence signal intensity from bone cavities 145um below the surface. The AO-enhanced microscope provides cellular level images of mouse bone marrow at depths exceeding those achievable without AO.

Paper Details

Date Published: 14 February 2011
PDF: 8 pages
Proc. SPIE 7931, MEMS Adaptive Optics V, 79310H (14 February 2011); doi: 10.1117/12.875596
Show Author Affiliations
Yaopeng Zhou, Abbott Labs. (United States)
Thomas Bifano, Boston Univ. (United States)
Charles Lin, Wellman Ctr. for Photomedicine, Boston Univ. (United States)

Published in SPIE Proceedings Vol. 7931:
MEMS Adaptive Optics V
Scot S. Olivier; Thomas G. Bifano; Joel A. Kubby, Editor(s)

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