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Proceedings Paper

ALA-induced PpIX spectroscopy for brain tumor image-guided surgery
Author(s): Pablo A. Valdes; Frederic Leblond; Anthony Kim; Brent T. Harris; Brian C. Wilson; Keith D. Paulsen; David W. Roberts
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Paper Abstract

Maximizing the extent of brain tumor resection correlates with improved survival and quality of life outcomes in patients. Optimal surgical resection requires accurate discrimination between normal and abnormal, cancerous tissue. We present our recent experience using quantitative optical spectroscopy in 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence-guided resection. Exogenous administration of ALA leads to preferential accumulation in tumor tissue of the fluorescent compound, PpIX, which can be used for in vivo surgical guidance. Using the state of the art approach with a fluorescence surgical microscope, we have been able to visualize a subset of brain tumors, but the sensitivity and accuracy of fluorescence detection for tumor tissue with this system are low. To take full advantage of the biological selectivity of PpIX accumulation in brain tumors, we used a quantitative optical spectroscopy system for in vivo measurements of PpIX tissue concentrations. We have shown that, using our quantitative approach for determination of biomarker concentrations, ALA-induced PpIX fluorescence-guidance can achieve accuracies of greater than 90% for most tumor histologies. Here we show multivariate analysis of fluorescence and diffuse reflectance signals in brain tumors with comparable diagnostic performance to our previously reported quantitative approach. These results are promising, since they show that technological improvements in current fluorescence-guided surgical technologies and more biologically relevant approaches are required to take full advantage of fluorescent biomarkers, achieve better tumor identification, increase extent of resection, and subsequently, lead to improve survival and quality of life in patients.

Paper Details

Date Published: 17 February 2011
PDF: 6 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78833P (17 February 2011); doi: 10.1117/12.875016
Show Author Affiliations
Pablo A. Valdes, Thayer School of Engineering (United States)
Dartmouth Medical School (United States)
Frederic Leblond, Thayer School of Engineering (United States)
Anthony Kim, Univ. Health Network (Canada)
Brent T. Harris, Georgetown Univ. Medical Ctr. (United States)
Brian C. Wilson, Univ. Health Network (Canada)
Keith D. Paulsen, Thayer School of Engineering (United States)
David W. Roberts, Dartmouth Medical School (United States)
Dartmouth Hitchcock Medical Ctr. (United States)

Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory M.D.; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg M.D.; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong M.D.; Justus F. R. Ilgner M.D.; Bodo E. Knudsen M.D.; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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