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Proceedings Paper

Combining multiphoton and CARS microscopy for skin imaging
Author(s): H. G. Breunig; M. Weinigel; J. Lademann; W. Sterry; I. Latka; B. Dietzek; J. Popp; K. König
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Paper Abstract

We present combined coherent anti Stokes Raman scattering and two-photon excited fluorescence microscopy, for label-free in vitro and in vivo imaging of skin with both chemical discrimination and subcellular resolution. In particular, we show how CARS microscopy is utilized to image lipid-rich structures inside the skin, preparing for a combined multiphoton and CARS imaging modality for biomedical research and skin imaging. As a further potential application the detection of topically applied oil on the skin is presented.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031A (11 February 2011); doi: 10.1117/12.874969
Show Author Affiliations
H. G. Breunig, JenLab GmbH (Germany)
M. Weinigel, JenLab GmbH (Germany)
J. Lademann, Charité (Germany)
W. Sterry, Charité (Germany)
I. Latka, IPHT, Univ. of Jena (Germany)
B. Dietzek, IPHT, Univ. of Jena (Germany)
J. Popp, IPHT, Univ. of Jena (Germany)
K. König, JenLab GmbH (Germany)
Saarland Univ. (Germany)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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