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Proceedings Paper

Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy
Author(s): YongKeun Park; Catherine Best; Thorsten Auth; Nir S. Gov; Samuel Safran; Gabriel Popescu
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Paper Abstract

We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations.

Paper Details

Date Published: 22 February 2011
PDF: 9 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021M (22 February 2011); doi: 10.1117/12.874768
Show Author Affiliations
YongKeun Park, KAIST (Korea, Republic of)
Catherine Best, Univ. of Illinois at Urbana-Champaign (United States)
Thorsten Auth, Institute for Complex Systems (Germany)
Nir S. Gov, Weizmann Institute of Science (Israel)
Samuel Safran, Weizmann Institute of Science (Israel)
Gabriel Popescu, Univ. of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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