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Proceedings Paper

Optical coherence tomography speckle decorrelation for detecting cell death
Author(s): Golnaz Farhat; Adrian Mariampillai; Victor X. D. Yang; Gregory J. Czarnota; Michael C. Kolios
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Paper Abstract

We present a dynamic light scattering technique applied to optical coherence tomography (OCT) for detecting changes in intracellular motion caused by cellular reorganization during apoptosis. We have validated our method by measuring Brownian motion in microsphere suspensions and comparing the measured values to those derived based on particle diffusion calculated using the Einstein-Stokes equation. Autocorrelations of OCT signal intensities acquired from acute myeloid leukemia cells as a function of treatment time demonstrated a significant drop in the decorrelation time after 24 hours of cisplatin treatment. This corresponded with nuclear fragmentation and irregular cell shape observed in histological sections. A similar analysis conducted with multicellular tumor spheroids indicated a shorter decorrelation time in the spheroid core relative to its edges. The spheroid core corresponded to a region exhibiting signs of cell death in histological sections and increased backscatter intensity in OCT images.

Paper Details

Date Published: 11 February 2011
PDF: 10 pages
Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 790710 (11 February 2011); doi: 10.1117/12.874682
Show Author Affiliations
Golnaz Farhat, Univ. of Toronto (Canada)
Sunnybrook Health Sciences Ctr. (Canada)
Adrian Mariampillai, Ryerson Univ. (Canada)
Ontario Cancer Institute (Canada)
Victor X. D. Yang, Sunnybrook Health Sciences Ctr. (Canada)
Ryerson Univ. (Canada)
Gregory J. Czarnota, Univ. of Toronto (Canada)
Sunnybrook Health Sciences Ctr. (Canada)
Michael C. Kolios, Univ. of Toronto (Canada)
Ryerson Univ. (Canada)

Published in SPIE Proceedings Vol. 7907:
Biomedical Applications of Light Scattering V
Adam P. Wax; Vadim Backman, Editor(s)

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