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Proceedings Paper

Tumor cell differentiation by marker free fluorescence microscopy
Author(s): Herbert Schneckenburger; Petra Weber; Michael Wagner; Marco Brantsch; Philipp Biller; Petra Kioschis; Waltraud Kessler
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Paper Abstract

Autofluorescence and Raman spectra, images and decay kinetics of U251-MG glioblastoma cells prior and after activation of tumor suppressor genes are compared. While phase contrast images and fluorescence patterns of the tumor (control) cells and the less malignant cells are similar, differences can be deduced from autofluorescence spectra and decay times. In particular, upon excitation around 375nm, the fluorescence ratio of the protein bound and the free coenzyme NADH depends on the state of malignancy. Slight differences are also observed in Raman spectra of these cell lines, in particular at wave numbers around 970 cm-1. While larger numbers of fluorescence and Raman spectra are evaluated by the method of multivariate data analysis, additional information is obtained from spectral images and fluorescence lifetime images (FLIM).

Paper Details

Date Published: 10 February 2011
PDF: 7 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020D (10 February 2011); doi: 10.1117/12.874655
Show Author Affiliations
Herbert Schneckenburger, Hochschule Aalen (Germany)
Petra Weber, Hochschule Aalen (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Marco Brantsch, Hochschule Aalen (Germany)
Philipp Biller, Hochschule Aalen (Germany)
Petra Kioschis, Hochschule Mannheim (Germany)
Waltraud Kessler, Steinbeis-Hochschule Berlin (Germany)

Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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