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Proceedings Paper

Developments of pulse-laser-assist optical tweezers (PLAT) for in vivo manipulation
Author(s): Saki Maeda; Tadao Sugiura; Kotaro Minato
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Paper Abstract

Optical tweezers is a technique to trap and to manipulate micron sized objects under a microscope by radiation pressure force exerted by a laser beam. Optical tweezers has been utilized for single-molecular measurements of force exerted by molecular interactions and for cell palpation. To extend applications of optical tweezers we have developed a novel optical tweezers system combined with a pulse laser. We utilize a pulsed laser (Q-switched Nd: YAG laser, wavelength of 1064 nm) to assist manipulations by conventional optical tweezers achieved by a continuous wave (CW) laser. The pulsed laser beam is introduced into the same optics for conventional optical tweezers. In principle, instantaneous radiation force is proportional to instantaneous power of laser beam. As a result, pulsed laser beam generates strong instantaneous force on an object to be manipulated. If the radiation force becomes strong enough to get over an obstacle structure and/or to be released from adhesion, the object will be free from these difficulties. We have named this technique as Pulse Laser beam Assisted optical Tweezers (PLAT). We have successfully demonstrated to manipulate objects surface on a living cell for "in vivo manipulation."

Paper Details

Date Published: 11 February 2011
PDF: 10 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021Y (11 February 2011); doi: 10.1117/12.874647
Show Author Affiliations
Saki Maeda, Nara Institute of Science and Technology (Japan)
Tadao Sugiura, Nara Institute of Science and Technology (Japan)
Kotaro Minato, Nara Institute of Science and Technology (Japan)

Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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