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Proceedings Paper

Non-invasive imaging and monitoring of rodent retina using simultaneous dual-band optical coherence tomography
Author(s): Peter Cimalla; Anke Burkhardt; Julia Walther; Aline Hoefer; Dierk Wittig; Richard Funk; Edmund Koch
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Paper Abstract

Spectral domain dual-band optical coherence tomography for simultaneous imaging of rodent retina in the 0.8 μm and 1.3 μm wavelength region and non-invasive monitoring of the posterior eye microstructure in the field of retinal degeneration research is demonstrated. The system is illuminated by a supercontinuum laser source and allows three-dimensional imaging with high axial resolution better than 3.8 μm and 5.3 μm in tissue at 800 nm and 1250 nm, respectively, for precise retinal thickness measurements. A fan-shaped scanning pattern with the pivot point close to the eye's pupil and a contact lens are applied to obtain optical access to the eye's fundus. First in vivo experiments in a RCS (royal college of surgeons) rat model with gene-related degeneration of the photoreceptor cells show good visibility of the retinal microstructure with sufficient contrast for thickness measurement of individual retinal layers. An enhanced penetration depth at 1250 nm is clearly identifiable revealing sub-choroidal structures that are not visible at 800 nm. Furthermore, additional simultaneous imaging at 1250 nm improves image quality by frequency compounding speckle noise reduction. These results are encouraging for time course studies of the rodent retina concerning its development related to disease progression and treatment response.

Paper Details

Date Published: 5 February 2011
PDF: 7 pages
Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 788909 (5 February 2011);
Show Author Affiliations
Peter Cimalla, Dresden Univ. of Technology (Germany)
Anke Burkhardt, Dresden Univ. of Technology (Germany)
Julia Walther, Dresden Univ. of Technology (Germany)
Aline Hoefer, Dresden Univ. of Technology (Germany)
Dierk Wittig, Dresden Univ. of Technology (Germany)
Richard Funk, Dresden Univ. of Technology (Germany)
Edmund Koch, Dresden Univ. of Technology (Germany)

Published in SPIE Proceedings Vol. 7889:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

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