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Proceedings Paper

Transillumination of subcutaneous adipose tissues using near-infrared hyperspectral imaging in the 1100-1800 nm wavelength range
Author(s): K. Ishii; A. Kitayabu; Y. Kobayashi; N. Honda; K. Awazu
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Paper Abstract

Hyperspectral imaging (HSI) is a chemical imaging modality with spectroscopic information. This technique was often used in agricultural or pharmaceutical industries. But there have been a few reports for clinical medical applications. In near-infrared (NIR) wavelength region, the significant absorption peaks are often observed by the overtone of midinfrared molecular vibration. In addition, NIR light has a high penetration because of low scattering and less absorption by water or protein. In this study, we constructed the NIR-HSI system and the high-contrast subcutaneous adipose tissue imaging was conducted in-vitro. In the absorption spectra which are obtained by our NIR-HSI system, the characteristic absorption bands were observed around 1200 nm and 1700 nm. In the processed images using these wavelength bands, subcutaneous adipose tissue was observed through a skin. In a hyperspectral image by another processing using all wavelengths, a high-contrast image of subcutaneous adipose tissue is also obtained. NIR-HSI system is a powerful diagnostic technique for adipose tissues distribution and their morphological change on/inside a tissue.

Paper Details

Date Published: 28 February 2011
PDF: 4 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021X (28 February 2011); doi: 10.1117/12.874540
Show Author Affiliations
K. Ishii, Osaka Univ. (Japan)
A. Kitayabu, Osaka Univ. (Japan)
Y. Kobayashi, Osaka Univ. (Japan)
N. Honda, Osaka Univ. (Japan)
K. Awazu, Osaka Univ. (Japan)
Univ. of Fukui (Japan)
Kyoto Univ. (Japan)

Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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