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Proceedings Paper

Functional transcranial photoacoustic micro-imaging of mouse cerebrovascular cross-section and hemoglobin oxygenation changes during forepaw electrical stimulation
Author(s): Lun-De Liao; You-Yin Chen; Chin-Teng Lin; Jyh-Yeong Chang; Meng-Lin Li
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Paper Abstract

In this study, we report on using a 50-MHz functional photoacoustic microscopy (PAM) to transcranially image the cross-section and hemoglobin oxygenation (SO2) changes of single mouse cortical vessels in response to left forepaw electrical stimulation. Three difference levels of the cortical vessels (i.e., with different-sized diameters of 350, 100 and 55 μm) on activated regions were marked to measure their functional cross-section and SO2 changes as a function of time. Electrical stimulation of the mouse left forelimb was applied to evoke functional changes in vascular dynamics of the mouse somatosensory cortex. The applied current pulses were with a pulse frequency of 3 Hz, pulse duration of 0.2 ms, and pulse amplitude of 2 mA. The cerebrovascular cross-section changes, which indicate changes in cerebral blood volume (CBV), were probed by images acquired at 570 nm, a hemoglobin isosbestic point, while SO2 changes were monitored by the derivatives of 560-nm images normalized to 570-nm ones. The results show that vessel diameter and SO2 were significantly dilated and increased when compared with those of the controlled ones. In summary, the PAM shows its promise as a new imaging modality for transcranially functional quantification of single vessel diameter (i.e., CBV) and SO2 changes without any contrast agents applied during stimulation.

Paper Details

Date Published: 18 February 2011
PDF: 7 pages
Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78992X (18 February 2011); doi: 10.1117/12.874449
Show Author Affiliations
Lun-De Liao, National Chiao Tung Univ. (Taiwan)
You-Yin Chen, National Chiao Tung Univ. (Taiwan)
Chin-Teng Lin, National Chiao Tung Univ. (Taiwan)
Jyh-Yeong Chang, National Chiao Tung Univ. (Taiwan)
Meng-Lin Li, National Tsing Hua Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7899:
Photons Plus Ultrasound: Imaging and Sensing 2011
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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