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Proceedings Paper

Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD
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Paper Abstract

Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness, conformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with good statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition speed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in throughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS 1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.

Paper Details

Date Published: 28 February 2011
PDF: 8 pages
Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 790503 (28 February 2011); doi: 10.1117/12.874238
Show Author Affiliations
Ryan A. Colyer, Univ. of California, Los Angeles (United States)
Giuseppe Scalia, Univ. of California, Los Angeles (United States)
Federica A. Villa, Politecnico di Milano (Italy)
Fabrizio Guerrieri, Politecnico di Milano (Italy)
Simone Tisa, Micro Photon Devices (Italy)
Franco Zappa, Politecnico di Milano (Italy)
Sergio Cova, Politecnico di Milano (Italy)
Shimon Weiss, Univ. of California, Los Angeles (United States)
Xavier Michalet, Univ. of California, Los Angeles (United States)

Published in SPIE Proceedings Vol. 7905:
Single Molecule Spectroscopy and Imaging IV
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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