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Proceedings Paper

A pilot characterization of quantitative time-domain fluorescence diffuse optical tomography
Author(s): Jiao Li; Feng Gao; Xi Yi; Xin Wang; Linhui Wu; Pingping Zhu; Limin Zhang; Huijuan Zhao
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Paper Abstract

Quantitative measurements of fluorescent parameters have merited great interest lately for near-infrared fluorescence diffuse optical tomography - the efficient small animal imaging tool. We present a two-dimensional image reconstruction method for time-domain fluorescence diffuse optical tomography, which employs the analytical solution to the Laplace-transformed time-domain photon-diffusion equation to construct the inverse model and introduces a pair of real-domain transform-factors to effectively separate the fluorescent yield and lifetime parameters from the algebraic reconstruction technique solutions to the resultant linear inversions. By use of a specifically designed a multi-channel time-correlated single photon counting system and a normalized Born formulation for the inversion, the proposed scheme in a circular domain is experimentally validated using small-animal-sized cylindrical phantoms that embed several fluorescent targets made from 1%-Intralipid solution and differently contrasting fluorescent agents, where the time-resolved excitation and fluorescence signals are measured on the boundary. The results show that the approach retrieves the positions and shapes of the targets with a reasonable accuracy and simultaneously achieve quantitative reconstruction of the fluorescent yield and lifetime.

Paper Details

Date Published: 1 March 2011
PDF: 11 pages
Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 78961G (1 March 2011); doi: 10.1117/12.874102
Show Author Affiliations
Jiao Li, Tianjin Univ. (China)
Feng Gao, Tianjin Univ. (China)
Tianjin Key Lab. of Biomedical Detecting Techniques and Instruments (China)
Xi Yi, Tianjin Univ. (China)
Xin Wang, Tianjin Univ. (China)
Linhui Wu, Tianjin Univ. (China)
Pingping Zhu, Tianjin Univ. (China)
Limin Zhang, Tianjin Univ. (China)
Tianjin Key Lab. of Biomedical Detecting Techniques and Instruments (China)
Huijuan Zhao, Tianjin Univ. (China)

Published in SPIE Proceedings Vol. 7896:
Optical Tomography and Spectroscopy of Tissue IX
Bruce Jason Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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