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Proceedings Paper

Time-lapsed integrated Raman and angular-scattering microscopy of single cells
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Paper Abstract

Integrated Raman- and Angular-scatteringMicroscopy (IRAM) combines two light scattering techniques to make chemical and morphological measurements of intact, single cells without the use of external labeling. IRAM has previously demonstrated its ability to differentiate between activated and non-activated CD8+ T cells based on both chemical and morphological differences. Activated cells showed an increase in protein and lipid content as well as an increase in the size and number of 0.5-1.0 μm diameter scatterers (likely lysosomes). Recent improvements to the IRAM system enable studies over an extended period of time. The applications of IRAM to chemical and structural changes of single cells during biological processes and treatments will be discussed.

Paper Details

Date Published: 11 February 2011
PDF: 8 pages
Proc. SPIE 7907, Biomedical Applications of Light Scattering V, 79070X (11 February 2011); doi: 10.1117/12.873906
Show Author Affiliations
Dustin W. Shipp, Univ. of Rochester (United States)
Andrew J. Berger, Univ. of Rochester (United States)

Published in SPIE Proceedings Vol. 7907:
Biomedical Applications of Light Scattering V
Adam P. Wax; Vadim Backman, Editor(s)

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