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Proceedings Paper

Optically sectioned imaging by oblique plane microscopy
Author(s): Sunil Kumar; Ziduo Lin; Alex R. Lyon; Ken T. MacLeod; Chris Dunsby
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Paper Abstract

Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. The first OPM results obtained using a high NA water immersion lens on a commercially available inverted microscope frame are presented, together with a measurement of the achievable optical resolution.

Paper Details

Date Published: 28 February 2011
PDF: 4 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040X (28 February 2011); doi: 10.1117/12.873471
Show Author Affiliations
Sunil Kumar, Imperial College London (United Kingdom)
Ziduo Lin, Imperial College London (United Kingdom)
Alex R. Lyon, Imperial College London (United Kingdom)
Ken T. MacLeod, Imperial College London (United Kingdom)
Chris Dunsby, Imperial College London (United Kingdom)

Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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