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Proceedings Paper

Fluorescence lifetime imaging of skin cancer
Author(s): Rakesh Patalay; Clifford Talbot; Ian Munro; Hans Georg Breunig; Karsten König; Yuri Alexandrov; Sean Warren; Mark A. A. Neil; Paul M. W. French; Anthony Chu; Gordon W. Stamp; Chris Dunsby
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Paper Abstract

Fluorescence intensity imaging and fluorescence lifetime imaging microscopy (FLIM) using two photon microscopy (TPM) have been used to study tissue autofluorescence in ex vivo skin cancer samples. A commercially available system (DermaInspect®) was modified to collect fluorescence intensity and lifetimes in two spectral channels using time correlated single photon counting and depth-resolved steady state measurements of the fluorescence emission spectrum. Uniquely, image segmentation has been used to allow fluorescence lifetimes to be calculated for each cell. An analysis of lifetime values obtained from a range of pigmented and non-pigmented lesions will be presented.

Paper Details

Date Published: 17 February 2011
PDF: 7 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830A (17 February 2011); doi: 10.1117/12.873298
Show Author Affiliations
Rakesh Patalay, Imperial College London (United Kingdom)
Clifford Talbot, Imperial College London (United Kingdom)
Ian Munro, Imperial College London (United Kingdom)
Hans Georg Breunig, JenLab GmbH (Germany)
Karsten König, JenLab GmbH (Germany)
Yuri Alexandrov, Imperial College London (United Kingdom)
Sean Warren, Imperial College London (United Kingdom)
Mark A. A. Neil, Imperial College London (United Kingdom)
Paul M. W. French, Imperial College London (United Kingdom)
Anthony Chu, Imperial College Healthcare (United Kingdom)
Gordon W. Stamp, Imperial College London (United Kingdom)
The Royal Marsden Hospital (United Kingdom)
Chris Dunsby, Imperial College London (United Kingdom)

Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory M.D.; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg M.D.; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong M.D.; Justus F. R. Ilgner M.D.; Bodo E. Knudsen M.D.; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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