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Proceedings Paper

Microfluidic system for single cell sorting with optical tweezers
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Paper Abstract

A microfluidic system was developed and combined with optical tweezers for single cell sorting. This system consists of a glass chip of 300 μm thickness with an etched crosswise channel structure, a silicon layer for sealing and a PMMA substrate for tubular coupling. Selected cells are trapped and moved in perpendicular direction to the main flow for recovery in special reservoirs and further evaluation (e.g. by polymerase chain reaction, PCR). In addition, maximum light doses and exposure times for maintaining cell viability were determined.

Paper Details

Date Published: 24 November 2010
PDF: 5 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 73760M (24 November 2010); doi: 10.1117/12.871450
Show Author Affiliations
Thomas Bruns, Hochschule Aalen (Germany)
Laszlo Becsi, Hochschule Furtwangen (Germany)
Marc Talkenberg, Hochschule Aalen (Germany)
Michael Wagner, Hochschule Aalen (Germany)
Petra Weber, Hochschule Aalen (Germany)
Ulrich Mescheder, Hochschule Furtwangen (Germany)
Herbert Schneckenburger, Hochschule Aalen (Germany)

Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences
Matti Kinnunen; Risto Myllylä, Editor(s)

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