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Proceedings Paper

Sum frequency generation spectroscopy of amyloid fibrils and oligomers at air/water interface
Author(s): Gediminas Niaura; Rima Budvytyte; Zenonas Kuprionis; Gintaras Valincius
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Paper Abstract

Structure of beta-amyloid oligomers and fibrils at air/water interface was studied by sum frequency generation (SFG) spectroscopy. It was found that both small (1-3 nm) and large (4-10 nm) oligomers as well as fibrils adsorb in ordered fashion at air/water interface. Intense resonances from methyl group stretching vibrations at 2875 and 2937 cm-1 and "ice-like" structured water bands were characteristic for adsorbed fibrils. Similar spectral pattern was observed for large oligomers. However, small oligomers exhibited intense SFG resonance near 2912 cm-1 due to methylene or methine C-H stretching vibration. No "ice-like" water band was observed in this case. The possibility of discrimination between the small (toxic) oligomers, large (non-toxic) oligomers and fibrils was demonstrated.

Paper Details

Date Published: 24 November 2010
PDF: 7 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 73760Q (24 November 2010); doi: 10.1117/12.871042
Show Author Affiliations
Gediminas Niaura, Institute of Biochemistry (Lithuania)
Rima Budvytyte, Institute of Biochemistry (Lithuania)
Zenonas Kuprionis, EKSPLA Ltd. (Lithuania)
Gintaras Valincius, Institute of Biochemistry (Lithuania)

Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences
Matti Kinnunen; Risto Myllylä, Editor(s)

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