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Proceedings Paper

Light induced heme oxygenase 1 is suppressed by Bach1 in human skin keratinocytes
Author(s): Ruoqi Xu; Wanqian Liu; Jin Zhang; Li Yang; Linhong Deng; Rex M. Tyrrell; Julia Li Zhong
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Paper Abstract

Ultraviolet A (UVA) radiation is an oxidizing agent that strongly induces the heme oxygenase 1(HO-1) expression in cultured human skin fibroblasts, but weakly induces it in skin keratinocytes. Here, we report that low basal levels of HO- 1 and much higher basal levels of HO-2 protein were observed in keratinocytes compared with fibroblasts. Silencing of Bach1 strongly increased HO-1 levels in HaCaT transformed keratinocytes and these HO-1 levels were not further increased by either UVA irradiation or silencing of HO-2. This is consistent with the conclusion that high constitutive levels of HO-2 expression in keratinocytes are responsible for the resistance of these cells to HO-1 induction by UVA radiation and that Bach1 plays a predominant role in influencing the lack of HO-1 expression in keratinocytes. Bach1 inhibition reduced the 500 kJ/m2 UVA-induced cell damage by LDH membrane integrity and MTS viability assays. These results suggest that Bach1 inhibition protect against high dose of UVA irradiation induced damage in keratinocytes.

Paper Details

Date Published: 8 November 2010
PDF: 9 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450Z (8 November 2010); doi: 10.1117/12.870984
Show Author Affiliations
Ruoqi Xu, Chongqing Univ. (China)
Wanqian Liu, Chongqing Univ. (China)
Jin Zhang, Univ. of Bath (United Kingdom)
Li Yang, Chongqing Univ. (China)
Linhong Deng, Chongqing Univ. (China)
Rex M. Tyrrell, Univ. of Bath (United Kingdom)
Julia Li Zhong, Chongqing Univ. (China)

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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