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Proceedings Paper

Cell imaging techniques based on digital image plane holography
Author(s): Zhaoji Chen; Wendi Gong; Feifei Liu; Huaying Wang
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Paper Abstract

This paper has further studied the implementation methods and recording conditions of digital microscopic image plane holography (DMIPH). Two optical systems of DMIPH were built: one is recording hologram by using plane waves as reference light, the other is recording hologram by spherical reference light. Breast cancer cells and USAF resolution test target is used as tested samples in the experiment. Then the intensity distribution and three-dimensional shape information of the cells are got accurately. The experiment results show that DMIPH avoids the process of finding recording distance by using auto-focusing approach. The recording and reconstruction process of DMIPH is simple. Therefore DMIPH can be applied to the microscopic imaging of cells more effectively.

Paper Details

Date Published: 17 November 2010
PDF: 6 pages
Proc. SPIE 7848, Holography, Diffractive Optics, and Applications IV, 78482Z (17 November 2010); doi: 10.1117/12.870536
Show Author Affiliations
Zhaoji Chen, Handan College (China)
Wendi Gong, Hebei Univ. of Engineering (China)
Feifei Liu, Hebei Univ. of Engineering (China)
Huaying Wang, Hebei Univ. of Engineering (China)

Published in SPIE Proceedings Vol. 7848:
Holography, Diffractive Optics, and Applications IV
Yunlong Sheng; Chongxiu Yu; Linsen Chen, Editor(s)

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