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Proceedings Paper

Feasibility study for rapid determination of alanine aminotransferase concentration in whole blood by using visible and near infrared spectroscopy
Author(s): Furong Huang; Zhe Chen; Jianhui Yu; Shiping Li; Yunhan Luo; Shifu Zheng
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Paper Abstract

Visible and Near infrared spectroscopy was applied for the fast determination of alanine aminotransferase with whole blood. First, spectra of different thickness (0.5mm, 1mm, 2mm, 4mm ) were investigated to explore Optimal Optical Path for determination. The results show that the whole blood sample with 0.5mm thickness is more suitable for spectral analysis. And then Near infrared spectroscopy of 176 samples were collected. Multiplicative scatter correction and second-order differential method have been used to spectral pretreatment. Stepwise multiple linear regression method and partial least squares regression method have been employed to establish quantitative detection model to predict content of alanine aminotransferase in whole blood. The alanine aminotransferase measured presents best result in calibration and prediction by Near-Infrared Spectroscopy with partial least squares regression calibration model, and the calibration correlation coefficient, the standard error of calibration and the standard error of prediction are 0.98, 2.42 and 7.22 respectively.

Paper Details

Date Published: 8 November 2010
PDF: 8 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450O (8 November 2010); doi: 10.1117/12.870251
Show Author Affiliations
Furong Huang, Jinan Univ. (China)
Zhe Chen, Jinan Univ. (China)
Jianhui Yu, Jinan Univ. (China)
Shiping Li, Jinan Univ. (China)
Yunhan Luo, Jinan Univ. (China)
Shifu Zheng, The First Affiliated Hospital Jinan Univ. (China)

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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