Singlet oxygen (¹O₂) is widely considered to be the predominant cytotoxic agent for photodynamic therapy (PDT), and the ¹O₂ quantum yield is a key characteristic for the newly developed photosensitizers. In this study, ¹O₂ generation from the HiPorfin is monitored indirectly by using the fluorescence probe Singlet Oxygen Sensor Green (SOSG). The good linear relationship between the concentration of HiPorfin and the initial reaction rate of SOSG is observed, and the ¹O₂ quantum yield of HiPorfin is determined to be 0.124 ± 0.001, as compared to the standard reference of Rose Bengal. The results suggest that SOSG can be useful for ¹O₂ quantum yield determination for the potential photosensitizers in PDT.

Date Published: 8 November 2010
PDF: 6 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451J (8 November 2010); doi: 10.1117/12.870108

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)