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Proceedings Paper

Fluorescence lifetime imaging using multi-dimension time-correlated single photon counting method
Author(s): Cuixia Sheng; Heijing Tang
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Paper Abstract

The key technology of fluorescence lifetime imaging is introduced in this article. Time-correlated single photon counting (TCSPC) in Time-domain method is adopted to record fluorescence lifetime . The principle of TCSPC and the Multi-wavelength imaging method are introduced in the paper. The counting efficiency and the amount of information in the data can be increased by recording the fluorescence in several wavelength channels simultaneously. Multi-wavelength imaging was also successfully used to separate different chromophores in stead-state images.

Paper Details

Date Published: 8 November 2010
PDF: 5 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451V (8 November 2010); doi: 10.1117/12.868319
Show Author Affiliations
Cuixia Sheng, Shandong Univ. of Technology (China)
Heijing Tang, Shandong Univ. of Technology (China)

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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