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Proceedings Paper

An optical tweezers study of nanosecond duration DNA conformations through DNA-surface binding distance measurements
Author(s): Julea Vlassakis; Sujay Tyle; Tom Crawford; Jeremy Williams; Josie Weeks; Tom Kodger; Efraim Feinstein; Claudia Danilowicz; Vincent Coljee; Mara Prentiss
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Paper Abstract

Optical tweezers have been widely used to study DNA properties including time dependent changes in conformation; however, such studies have emphasized direct fluorescent observation of the conformations of dyed DNA molecules. In this work we explore DNA conformations that allow undyed DNA to link to spatially separated surfaces. In one set of experiments, we used optical tweezers to hold a polystyrene bead at a fixed distance from the sample capillary wall and measured the probability of the binding as a function of the separation between the polystyrene bead and the capillary, where the beads were fully confined in liquid. In a separate magnetic crystal experiment, we used magnetic forces to control the separation between magnetic beads in a hexagonal lattice at an air-water interface and measured the probability of linking to beads in the crystal. In both types of experiments peak binding occurs at a surface separation several times longer than the radius of gyration of the DNA. These experiments provide fundamental information on elusive, but significant DNA conformations, as well as technologically useful information on the probability of the DNA binding that will link two surfaces.

Paper Details

Date Published: 27 August 2010
PDF: 10 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77623F (27 August 2010); doi: 10.1117/12.868284
Show Author Affiliations
Julea Vlassakis, Smith College (United States)
Sujay Tyle, Harvard Univ. (United States)
Tom Crawford, Williams College (United States)
Jeremy Williams, Middlebury College (United States)
Josie Weeks, Harvard Univ. (United States)
Tom Kodger, Harvard Univ. (United States)
Efraim Feinstein, Harvard Univ. (United States)
Claudia Danilowicz, Harvard Univ. (United States)
Vincent Coljee, Harvard Univ. (United States)
Mara Prentiss, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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