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Proceedings Paper

Functional multilayer coated long period grating tuned in transition region for life science applications
Author(s): P. Pilla; V. Malachovská; A. Borriello; M. Giordano; L. Ambrosio; A. Cutolo; A. Cusano
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Paper Abstract

We report preliminary results on the development of multilayer coated long period gratings (LPGs) for life science applications. The dip-coating technique and a solvent/nonsolvent strategy were exploited to deposit double-layer polymeric film onto a LPG. A primary coating of atactic polystyrene was used as high refractive index layer to tune the working point of the device in the so-called transition region thus achieving remarkable surrounding medium refractive index sensitivity. A secondary layer of atactic poly(methyl methacrylate-co-methacrylic acid) containing functional carboxyl groups, characterized by a lower refractive index, was deposited onto the primary coating in order to have the desired functional groups on the surface of the device. Commonly used covalent immobilization procedure, NHS/EDC coupling method, was exploited to link streptavidin on the surface of the functionalized coated device. Finally, real-time detection of biotinylated bovine serum albumin affinity binding on immobilized streptavidin was performed by monitoring the shift of the LPG attenuation bands.

Paper Details

Date Published: 8 September 2010
PDF: 4 pages
Proc. SPIE 7653, Fourth European Workshop on Optical Fibre Sensors, 76531O (8 September 2010); doi: 10.1117/12.866188
Show Author Affiliations
P. Pilla, Univ. degli Studi del Sannio (Italy)
V. Malachovská, National Research Council (Italy)
A. Borriello, National Research Council (Italy)
M. Giordano, National Research Council (Italy)
L. Ambrosio, National Research Council (Italy)
A. Cutolo, Univ. degli Studi del Sannio (Italy)
A. Cusano, Univ. degli Studi del Sannio (Italy)

Published in SPIE Proceedings Vol. 7653:
Fourth European Workshop on Optical Fibre Sensors
José Luís Santos; Brian Culshaw; José Miguel López-Higuera; William N. MacPherson, Editor(s)

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