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Proceedings Paper

Dynamic complex optical fields for optical manipulation, 3D microscopy, and photostimulation of neurotransmitters
Author(s): Vincent Ricardo Daria; Christian Stricker; John Bekkers; Steve Redman; Hans Bachor
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Paper Abstract

We demonstrate a multi-functional system capable of multiple-site two-photon excitation of photo-sensitive compounds as well as transfer of optical mechanical properties on an array of mesoscopic particles. We use holographic projection of a single Ti:Sapphire laser operating in femtosecond pulse mode to show that the projected three-dimensional light patterns have sufficient spatiotemporal photon density for multi-site two-photon excitation of biological fluorescent markers and caged neurotransmitters. Using the same laser operating in continuous-wave mode, we can use the same light patterns for non-invasive transfer of both linear and orbital angular momentum on a variety of mesoscopic particles. The system also incorporates high-speed scanning using acousto-optic modulators to rapidly render 3D images of neuron samples via two-photon microscopy.

Paper Details

Date Published: 27 August 2010
PDF: 9 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621S (27 August 2010); doi: 10.1117/12.862388
Show Author Affiliations
Vincent Ricardo Daria, ARC Ctr. for Quantum Atom Optics (Australia)
The Australian National Univ. (Australia)
Christian Stricker, The Australian National Univ. (Australia)
John Bekkers, The Australian National Univ. (Australia)
Steve Redman, The Australian National Univ. (Australia)
Hans Bachor, ARC Ctr. for Quantum Atom Optics (Australia)

Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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